Foxtail Mosaic Virus-Induced Gene Silencing in Monocot Plants
نویسندگان
چکیده
منابع مشابه
Foxtail Mosaic Virus-Induced Gene Silencing in Monocot Plants.
Virus-induced gene silencing (VIGS) is a powerful technique to study gene function in plants. However, very few VIGS vectors are available for monocot plants. Here we report that Foxtail mosaic virus (FoMV) can be engineered as an effective VIGS system to induce efficient silencing of endogenous genes in monocot plants including barley (Hordeum vulgare L.), wheat (Triticum aestivum) and foxtail...
متن کاملFoxtail Mosaic Virus-Induced Gene Silencing in Monocot Plants1[OPEN]
Center for Plant Biology and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (N.L., K.X., Q.J., J.Z., T.C., X.W., H.L., Y.L.); Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China (X.D.); Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University,...
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Background:Potyvirus-based virus-induced gene silencing (VIGS) is used for knocking down the expression of a target gene in numerous plant species. Sugarcane mosaic virus (SCMV) is a monopartite, positive single strand RNA virus. Objectives:pBINTRA6 vector was modified by inserting a gene segment of SCMV in place of Tobacco rattle virus (TRV) genome part 1 (TRV1 or RNA1)...
متن کاملA Foxtail mosaic virus Vector for Virus-Induced Gene Silencing in Maize.
Plant viruses have been widely used as vectors for foreign gene expression and virus-induced gene silencing (VIGS). A limited number of viruses have been developed into viral vectors for the purposes of gene expression or VIGS in monocotyledonous plants, and among these, the tripartite viruses Brome mosaic virus and Cucumber mosaic virus have been shown to induce VIGS in maize (Zea mays). We de...
متن کاملVirus-induced gene silencing in plants.
Virus-induced gene silencing (VIGS) is a technology that exploits an RNA-mediated antiviral defense mechanism. In plants infected with unmodified viruses the mechanism is specifically targeted against the viral genome. However, with virus vectors carrying inserts derived from host genes the process can be additionally targeted against the corresponding mRNAs. VIGS has been used widely in plants...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 2016
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.16.00010